Journal: International Journal of Molecular Medicine
Article Title: Apelin-13 alleviates diabetic nephropathy by enhancing nitric oxide production and suppressing kidney tissue fibrosis
doi: 10.3892/ijmm.2021.5008
Figure Lengend Snippet: Apelin-13 alleviates the higher glucose-induced injury of endothelial cells of glomerular vessels. (A) Cell viability was detected with the Cell Counting Kit-8 assay. (B) hRGECs were detected with the lumen formation experiments (magnification, x4). (C) The expression of AT1R, eNOS, APJ, TGBR, E-cadherin and α-SMA in these cells was determined with the western blotting. * P<0.05, ** P<0.01 and *** P<0.001. AT1R, Ang II type 1 receptor; eNOS, endothelial nitric oxide synthase; APJ, angiotensin domain type 1 receptor-associated proteins; TGBR, transforming growth factor β receptor; α-SMA, α-smooth muscle actin.
Article Snippet: A human renal glomerular microvascular endothelial cell line (hRGEC) was obtained from the BioVector NTCC, Inc. and cultured in RPMI-1640 medium (Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (FBS) in humidified air at 37°C with 5% CO 2 .
Techniques: Cell Counting, Expressing, Western Blot